Download Pumps, channels, and transporters : methods of functional by Ronald J. Clarke, Mohammed A. A. Khalid PDF
By Ronald J. Clarke, Mohammed A. A. Khalid
Describes experimental equipment for investigating the functionality of pumps, channels and transporters
- Covers new rising analytical equipment used to review ion shipping membrane proteins akin to single-molecule spectroscopy
- Details quite a lot of electrophysiological suggestions and spectroscopic equipment used to research the functionality of ion channels, ion pumps and transporters
- Covers state-of-the artwork analytical ways to research ion pumps, channels, and transporters, and the place analytical chemistry could make additional contributions
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Describes experimental equipment for investigating the functionality of pumps, channels and transporters Covers new rising analytical equipment used to review ion shipping membrane proteins akin to single-molecule spectroscopy info quite a lot of electrophysiological suggestions and spectroscopic tools used to research the functionality of ion channels, ion pumps and transporters Covers state-of-the paintings analytical the way to learn ion pumps, channels, and transporters, and the place analytical chemistry could make extra contributions
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Extra resources for Pumps, channels, and transporters : methods of functional analysis
The crucial point is that photon absorption causes a massive drop in the pKa of the Schiff base, that is, a significant increase in acidity. In the dark, the pKa of the Schiff base is around 12 and thus protonated at neutral and even slightly basic pH. After photon absorption and the conversion of retinal to the 13‐cis state, the pKa of the Schiff base drops by 4–5 pH units, thus promoting the loss of a proton. The drop in pKa of the Schiff base is due to a change in its electrostatic environment caused by the protein’s conformational change, that is, the proximity to other charged amino acid side chains.
The most widely used substrate is ATP released from its inactive precursor, caged ATP, by a UV‐light flash induced photolysis. The transient current generated in response to the ATP‐ concentration jump is a transfer of positive charge through the Na+,K+‐ATPase in the membrane fragments, as has been shown in the first publications using this method [5, 21]. In the case of ion pumps that cannot be obtained in flat membrane fragments, which adsorb to a BLM, a modified experimental approach was developed.
52. S. Lorkowski, P. Cullen, ABCG subfamily of human ATP‐binding cassette proteins, Pure Appl. Chem. 74 (2002) 2057–2081. 53. J. Stefková, R. A. Hubácek, ATP‐binding cassette (ABC) transporters in human metabolism and diseases, Physiol. Res. 53 (2004) 235–243. 54. L. Galvani, De viribus electricitatis in motu musculari commentarius, Accademia delle Scienza, Bologna, 1791. 2 STUDY OF ION PUMP ACTIVITY USING BLACK LIPID MEMBRANES Hans‐Jürgen Apell1 and Valerij S. Sokolov2 Department of Biology, University of Constance, Constance, Germany A.