Bioengineering

Download Bioengineering of the Skin: Skin Imaging & Analysis, 2nd by Klaus-Peter Wilhelm, Peter Elsner, Enzo Berardesca, Howard PDF

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By Klaus-Peter Wilhelm, Peter Elsner, Enzo Berardesca, Howard I. Maibach

Spanning the various developments that experience taken position within the box because the First variation of this ebook used to be released, this moment version emphasizes the imaging of the outside in its entirety, instead of focusing exclusively on floor layers. the second one variation contains new chapters on applied sciences equivalent to in vivo confocal laser scanning microscopy, Raman spectroscopy, optical coherence tomography, nuclear magnetic imaging, high-resolution ultrasound, in vivo epidermis topometry, and multi-photon imaging of the surface.

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Extra info for Bioengineering of the Skin: Skin Imaging & Analysis, 2nd Edition (Dermatology: Clinical & Basic Science)

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0) for one hour. 4) and rinsing in CB, this piece was dehydrated in graded concentrations of alcohol, immersed in isoamylacetate, dried up in a critical point drying apparatus with CO2, and observed in a SEM (JSM-840) following gold coating in an ion sputter (JFC-1100). 4b), dehydrated in graded concentrations of alcohol and acetone, and embedded in EponTM. For observation by LM, 1-mm thick sections of the embedded specimens were made with a diamond knife in an ultramicrotome (MT-5000) and stained in a toluidine blue solution.

Bioeng Skin 1987; 3:319–333. 6. Ryan RL, Hing SAO, Theiler RF. A replica technique for the evaluation of human skin by scanning electron microscopy. J Cutan Pathol 1983; 10:262. 7. Mihara M. Scanning electron microscopy of skin surface and the internal structure of corneocyte in normal human skin. An application of the osmium-dimethyl sulfoxideosmium method. Arch Dermatol Res 1988; 280:293. 8. Harding CR, Scott IR. Histidine rich proteins (filaggrins). Structural and functional heterogeneity during epidermal differentiation.

Likewise, after application of a 20% salicylic acid solution in ethanol 4 El Gammal et al. FIGURE 4 Corneocytes from the volar forearm after application of a 20% salicylic acid solution in ethanol for 60 minutes. Obtained by tape stripping, stained with rhodamine B/methylene blue, 165Â. on the volar forearm for 60 minutes, we found many torn and folded corneocytes as well as disintegrated cells on tape stripping (Fig. 4). Strong soap solutions will also swell corneocytes on samples obtained by stripping.

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